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A new, sensitive method for enzyme kinetic studies of scarce glucosides.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00018065" target="_blank" >RIV/00216224:14310/06:00018065 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081707:_____/06:00042442

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    A new, sensitive method for enzyme kinetic studies of scarce glucosides.

  • Original language description

    We have developed a rapid, accurate and sensitive assay for glucose that enabled us to measure the kinetic parameters for a cytokinin-O-glucoside-specific beta-glucosidase towards its natural substrate. Both the beta-glucosidase reaction and the detection reaction occur simultaneously in solution. Measurement is continuous and the reaction is measured nine times during three seconds.

  • Czech name

    Nová citlivá metoda pro stanovení kinetických parametrů přirozených substrátů.

  • Czech description

    Vyvinuli jsme rychlou přesnou a citlivou metodu pro stanovení glukózy, což nám umožnilo stanovit kinetické parametry v reakci cytokinin-O-glukozid specifické beta-glukozidázy s přirozeným substrátem. Beta-glukozidázová reakce i detekční reakce probíhajísoučasně v jednom roztoku. Měření je kontinuální a a provádí se devětkrát v průběhu tří sekund.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS

  • ISSN

    0165-022X

  • e-ISSN

  • Volume of the periodical

    68

  • Issue of the periodical within the volume

    2006

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    9

  • Pages from-to

    55-63

  • UT code for WoS article

  • EID of the result in the Scopus database