A suggestion of electrochemical biosensor for study of Platinum(II)-DNA interactions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00019367" target="_blank" >RIV/00216224:14310/07:00019367 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/07:00107864 RIV/62157124:16270/07:00001311

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A suggestion of electrochemical biosensor for study of Platinum(II)-DNA interactions

Original language description

As a consequence of the employment of platinum based cytostatic drugs in tumor diseases treatment, it became necessary not only to detect them in biological samples but also to determine and study the Pt-DNA adducts, which is highly important during investigation of resistance on antitumor treatment. Thus, the main objective of this work was to study the interactions between DNA and cisplatin. Primarily, we studied changes in electrochemical behavior of cisplatin in the presence of different concentrations of sodium chloride using differential pulse voltammetry.We found out that the highest signal of cisplatin was measured in 0.75 M NaCl with detection limit of 100 pM. The current accepted opinion about platinum based cytostatics mechanism of action isthat the drugs induce their cytotoxic properties through binding to the nuclear DNA. That is why we followed by investigation of DNA interactions with cisplatin. For these purposes we have suggested a new biosensor, EDTA metallothionein

Czech name

Návrh elektrochemického biosenzoru pro studium interakcí Platina (II)-DNA

Czech description

Návrh elektrochemického biosenzoru pro studium interakcí Platina (II)-DNA

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

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Volume of the periodical

19

Issue of the periodical within the volume

2-3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

331-338

UT code for WoS article

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EID of the result in the Scopus database

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