Catalytic Electrochemical Analysis of Platinum in Pt-DNA Adducts

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10126274" target="_blank" >RIV/00216208:11310/12:10126274 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/12:PU98519 RIV/00216208:11130/12:8102 RIV/00064203:_____/12:8102 RIV/62156489:43210/12:43908627
Result on the web
<a href="http://www.electrochemsci.org/papers/vol7/7043072.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol7/7043072.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Catalytic Electrochemical Analysis of Platinum in Pt-DNA Adducts
Original language description
Using of platinum based cytostatic drugs in tumour diseases treatment results in the need to detect these drugs in biological samples and to determine and study the Pt-DNA adducts, which is highly important for investigation of cisplatin-resistance developed during anti-tumour treatment. The aim of this study was not only electrochemical detection of cisplatin with the use of formazone method but also preparation of platinum-DNA complex and platinum content detection in DNA. Primarily, we optimized manual electrochemical detection using differential pulse voltammetry (DPV) for PtCl2 and cisplatin. The detection detection limits (3 S/N) were estimated as 10 pM and 30 nM for PtCl2 and cisplatin, respectively. Further, we automated the optimized method and reached 50 pM detection limit for PtCl2, but the method was proved to be versatile also for detection of cisplatin, which was verified by determination of cisplatin added cell culture medium. The method was also used to monitor the cont
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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