Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00024585" target="_blank" >RIV/00216224:14310/08:00024585 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction
Original language description
The major aim of this work is to demonstrate the applicability of micellar electrokinetic capillary chromatography with SDS based pseudostationary phase for the screening of cytochrome P450 inhibitors. In contrast with the other capillary electrophoresismodes the cytochrome P450 reaction mixture thus could be used for the analysis without any pre-treatment. Cytochrome P450 2C9, one of the most important isoforms in human liver, was chosen as a model example for this study in combination with diclofenacas a probe substrate. The inhibitory effect on the given cytochrome P450 reaction was evaluated for two inhibitors with different inhibition potential strong inhibitor sulfaphenazole and moderate inhibitor ketoconazole. As a result 50 % inhibitory concentrations IC50 and inhibition constants Ki were evaluated; their values for both inhibitors were in a good agreement with the literature data determined by different methods.
Czech name
Studium inhibice cytochromů P450 pomocí MEKC
Czech description
Byla vypracována metoda pro studium inhibice cytochromů P450 pomocí MEKC:

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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