The study on the FnrP, NNR and NarR transcription regulator function in bacterium Paracoccus denitrificans at global proteome level
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00025023" target="_blank" >RIV/00216224:14310/08:00025023 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The study on the FnrP, NNR and NarR transcription regulator function in bacterium Paracoccus denitrificans at global proteome level
Original language description
Paracoccus denitrificans is a non-fermentative, facultatively autotrophic soil bacterium often studied in the field of bioenergetics. In this work, we aimed to analyze the role of three known transcription regulators FnrP, NNR and NarR (with described role as sensors for oxygen and for intermediates of denitrification pathway) in these adaptation processes in P. denitrificans at global proteome level. We analyzed protein composition of four P. denitrificans strains: wild type, FnrP-, NNR- and NarR- mutants grown aerobically, semiaerobically and semiaerobically with nitrate. Total cell lysate proteins were separated using 2-D PAGE, 36 gels in experiment were statistically evaluated and ca. 500 protein spots were analyzed using MALDI-MS/MS and LC-MS/MS.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GP203%2F07%2FP471" target="_blank" >GP203/07/P471: The study of molecular mechanisms of bacterial adaptation to environmental changes using proteomic techniques</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů