Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029725" target="_blank" >RIV/00216224:14310/09:00029725 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
čeština
Original language name
Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches
Original language description
The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three Fnr-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively.In this study, we aimed to discover the role of Fnr-family transcription regulators in the aerobic-semiaerobic switch at global level. Methods and Materials: We analyzed the protein composition of four Paracoccus denitrificans strains (wild type, FnrP-,NNRand NarR- mutant strains) grown aerobically, semiaerobically and semiearobically in the presence of nitrate using two-dimensional gel electropohoresis with MS/MS protein identification, with data validation at the transcript (qRT-PCR) and genome (FNR-binding sequences) levels. Results: Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins gene products were identified using mass spectrometry.
Czech name
Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches
Czech description
The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three Fnr-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively.In this study, we aimed to discover the role of Fnr-family transcription regulators in the aerobic-semiaerobic switch at global level. Methods and Materials: We analyzed the protein composition of four Paracoccus denitrificans strains (wild type, FnrP-,NNRand NarR- mutant strains) grown aerobically, semiaerobically and semiearobically in the presence of nitrate using two-dimensional gel electropohoresis with MS/MS protein identification, with data validation at the transcript (qRT-PCR) and genome (FNR-binding sequences) levels. Results: Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins gene products were identified using mass spectrometry.
Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GP203%2F07%2FP471" target="_blank" >GP203/07/P471: The study of molecular mechanisms of bacterial adaptation to environmental changes using proteomic techniques</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů