In Silico and In Vitro Mutagenesis of PA-IIL Lectin - Correlation with Structure-Function Analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00025133" target="_blank" >RIV/00216224:14310/08:00025133 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
In Silico and In Vitro Mutagenesis of PA-IIL Lectin - Correlation with Structure-Function Analysis
Original language description
Protein-carbohydrate interactions play a crucial role in recognition and adhesion of pathogen to host tissue cells in the first step of their invasion and infectivity. Pseudomonas aeruginosa is an opportunistic pathogen, responsible for numerous nosocomial infections in immunocompromised patients. The bacterium colonises patients with chronic lung diseases and its infections is fatal in cystic fibrosis patients. P. aeruginosa produces a fucose-binding lectin PA-IIL that plays a role in virulence and adhesion [1]. Similar lectins have been found in other opportunistic bacteria like Ralstonia solanacearum [2], Chromobacterium violaceaum [3] and Burkholderia cenocepacia [4], with different binding preferences despite only slight sequence changes. Mutations of the PA-IIL lectin were designed according to these differences, cloned/modelled and investigated both in vitro, and in silico [5,6]. Thermodynamics of binding using isothermal titration microcalorimetry helped to rationalise importan
Czech name
In Silico and In Vitro Mutageneze lektinu PA-IIL - Korelace se strukturně-funkční analýzou
Czech description
In Silico and In Vitro Mutageneze lektinu PA-IIL - Korelace se strukturně-funkční analýzou

Project
<a href="/en/project/GA303%2F06%2F0570" target="_blank" >GA303/06/0570: Structure-function studies on lectins and adhesins from microbial patogens</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the Eighth Triennial Congress of the World Association of Theoretical and Computational Chemists (WATOC 2008)
ISBN
978-0-646-50151-2
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
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Publisher name
World Association of Theoretical and Computational Chemists
Place of publication
Sydney
Event location
Sydney
Event date
Jan 1, 2008
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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