Transactivation by temperature dependent p53 mutants in yeast and human cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00044309" target="_blank" >RIV/00216224:14310/10:00044309 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Transactivation by temperature dependent p53 mutants in yeast and human cells
Original language description
In response to stress signals, p53 controls essential cell functions by regulating expression of its target genes. Full or partial loss of the p53 function in cancer cells usually results from mutations of the p53 gene. Some of them are temperature-dependent, allowing reactivation of the p53 function in certain temperature.We analyzed transactivation of p21-, bax- and mdm2 genes by 23 temperature-dependent p53 mutants in transiently transfected human lung H1299 cells (p53-null) by luciferase reporter assay.We confirmed temperature-dependency and discriminative character of the most p53 mutants and stratified them into four functional groups. Despite the differences of yeast and human cells, they allowed similar transactivation rates to the p53 mutants,thus providing evidence that functional analysis of separated alleles in yeast is valuable tool for assessment of the human p53 status.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů