Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00054000" target="_blank" >RIV/00216224:14310/11:00054000 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping
Original language description
Background: Bifidobacteria represent an important part of human gastrointestinal tract maintaining and balancing proper intestinal microflora. Their correct identification is crucial for taxonomical, epidemiological and clinical purposes. Objectives: Theaim of this work was to evaluate capabilities of automated ribotyping for identification of bifidobacterial isolates from human intestine. Methods: A set of 70 Bifidobacterium strains originating from intestinal mucous tissue samples from children patients was identified by MALDI-TOF MS performed on an Ultraflex III instrument (Bruker Daltonik) and further characterized using automated ribotyping performed by the RiboPrinter system (DuPont Qualicon). Results: MALDI-TOF MS analysis enabled reliable identification (BioTyper log(score) > 2.3) of Bifidobacterium longum (36 strains), Bifidobacterium adolescentis (23), Bifidobacterium catenulatum (7), Bifidobacterium breve (3) and Bifidobacterium animalis (1).
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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