Identification and determination of relatedness of lactobacilli using different DNA amplification methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00060656" target="_blank" >RIV/00216224:14310/12:00060656 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/12:PU99147
Result on the web
<a href="http://dx.doi.org/10.2478/s11696-012-0206-7" target="_blank" >http://dx.doi.org/10.2478/s11696-012-0206-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/s11696-012-0206-7" target="_blank" >10.2478/s11696-012-0206-7</a>
Alternative languages
Result language
angličtina
Original language name
Identification and determination of relatedness of lactobacilli using different DNA amplification methods
Original language description
Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/2B06053" target="_blank" >2B06053: New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemical Papers
ISSN
0366-6352
e-ISSN
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Volume of the periodical
66
Issue of the periodical within the volume
9
Country of publishing house
PL - POLAND
Number of pages
10
Pages from-to
842-851
UT code for WoS article
000305563300007
EID of the result in the Scopus database
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