Differences in crystallization of two LinB variants from Sphingobium japonicum UT26
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00066680" target="_blank" >RIV/00216224:14310/13:00066680 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/13:00398267 RIV/60076658:12520/13:43885237
Result on the web
<a href="http://dx.doi.org/10.1107/S1744309113002467" target="_blank" >http://dx.doi.org/10.1107/S1744309113002467</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S1744309113002467" target="_blank" >10.1107/S1744309113002467</a>
Alternative languages
Result language
angličtina
Original language name
Differences in crystallization of two LinB variants from Sphingobium japonicum UT26
Original language description
Haloalkane dehalogenases are microbial enzymes that convert a broad range of halogenated aliphatic compounds to their corresponding alcohols by the hydrolytic mechanism. These enzymes play an important role in the biodegradation of various environmentalpollutants. Haloalkane dehalogenase LinB isolated from a soil bacterium Sphingobium japonicum UT26 has a relatively broad substrate specificity and can be applied in bioremediation and biosensing of environmental pollutants. The LinB variants presented here, LinB32 and LinB70, were constructed with the goal of studying the effect of mutations on enzyme functionality. In the case of LinB32 (L117W), the introduced mutation leads to blocking of the main tunnel connecting the deeply buried active site withthe surrounding solvent. The other variant, LinB70 (L44I, H107Q), has the second halide-binding site in a position analogous to that in the related haloalkane dehalogenase DbeA from Bradyrhizobium elkanii USDA94.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Crystallographica
ISSN
1744-3091
e-ISSN
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Volume of the periodical
69
Issue of the periodical within the volume
Pt 3
Country of publishing house
US - UNITED STATES
Number of pages
4
Pages from-to
284-287
UT code for WoS article
000316745000014
EID of the result in the Scopus database
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