High Frequency of Temperature-Sensitive Mutants of p53 in Glioblastoma

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00068188" target="_blank" >RIV/00216224:14310/13:00068188 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s12253-012-9596-7" target="_blank" >http://dx.doi.org/10.1007/s12253-012-9596-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12253-012-9596-7" target="_blank" >10.1007/s12253-012-9596-7</a>

Result language
angličtina
Original language name
High Frequency of Temperature-Sensitive Mutants of p53 in Glioblastoma
Original language description
Glioblastoma is the most common and the most aggressive type of brain cancer. Aberrations of the RTK/RAS/PI3K-, p53-, and RB cell signaling pathways were recognized as a core requirement for pathogenesis of glioblastoma. The p53 tumor suppressor functions as a transcription factor transactivating expression of its target genes in response to various stress stimuli. We determined the p53 status in 36 samples of glioblastoma by functional analyses FASAY and split assay. Seventeen p53 mutations were detected and further analyzed by cDNA and gDNA sequencing in 17 patients (47.2 %). Fifteen (88.2 %) of the mutations were missense mutations causing amino acid substitutions, seven of them exhibited temperature-sensitivity. Two mutations were determined as short deletions, one of them causing formation of premature termination codon in position 247. Fluorescent in situ hybridization revealed the loss of the p53-specific 17p13.3 locus in four of 33 analyzed samples (12 %).
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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