Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00107096" target="_blank" >RIV/00216224:14310/13:00107096 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/13:00213116 RIV/00216305:26620/13:PU104593 RIV/62157124:16370/13:43871833
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738958/pdf/viruses-05-01719.pdf" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738958/pdf/viruses-05-01719.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/v5071719" target="_blank" >10.3390/v5071719</a>
Alternative languages
Result language
angličtina
Original language name
Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene
Original language description
Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads (R) with covalently bound oligo (dT)(25) were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual probes hybridized to target molecules specifically with efficiency higher than 60%. The obtained signals identified mutations present in the sequence. Suggested experimental procedure allows obtaining further information from the redox signals of nucleic acids. Moreover, the used biosensor exhibits sequence specificity and low limits of detection of subnanogram quantities of target nucleic acids.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
VIRUSES-BASEL
ISSN
1999-4915
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
21
Pages from-to
1719-1739
UT code for WoS article
000322172200009
EID of the result in the Scopus database
2-s2.0-84880388877