Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F13%3APU104593" target="_blank" >RIV/00216305:26620/13:PU104593 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/13:00213116 RIV/62157124:16370/13:43871833 RIV/00216224:14310/13:00107096

Result on the web

<a href="https://www.mdpi.com/1999-4915/5/7/1719" target="_blank" >https://www.mdpi.com/1999-4915/5/7/1719</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/v5071719" target="_blank" >10.3390/v5071719</a>

Result language

angličtina

Original language name

Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene

Original language description

Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads with covalently bound oligo (dT)25 were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual probes hybridized to target molecules specifically with efficiency higher than 60%. The obtained signals identified mutations present in the sequence. Suggested experimental procedure allows obtaining further information from the redox signals of nucleic acids. Moreover, the used biosensor exhibits sequence specificity and low limits of detection of subnanogram quantities of target nucleic acids

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

VIRUSES-BASEL

ISSN

1999-4915

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

21

Pages from-to

1719-1739

UT code for WoS article

000322172200009

EID of the result in the Scopus database

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