Combination of on-line capillary electrophoretic assay with mass spectrometry detection for the study of drug metabolism by cytochromes P450
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080656" target="_blank" >RIV/00216224:14310/15:00080656 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201400394" target="_blank" >http://dx.doi.org/10.1002/elps.201400394</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201400394" target="_blank" >10.1002/elps.201400394</a>
Alternative languages
Result language
angličtina
Original language name
Combination of on-line capillary electrophoretic assay with mass spectrometry detection for the study of drug metabolism by cytochromes P450
Original language description
A new CE-MS method with enzymatic reaction inside the capillary was developed for the study of drug metabolism by cytochromes P450. This automated method, based on the transverse diffusion of laminar flow profiles methodology, is comprised of the injection of substrates and enzyme, their mixing, incubation and separation of the reaction products, all performed by CE, and their detection, identification and quantification by MS. The developed and validated method was finally used to conduct a kinetic study of cytochrome P450 isoform 2C9 or human liver microsomes with diclofenac in order to demonstrate its practical functionality. All the estimated kinetic values ? apparent Michaelis constants and apparent maximum reaction velocities were in agreement with literature data obtained using other techniques. In addition, the consumption of reactants was in the tens of nL per analysis. The method?s usability was further demonstrated on tolbutamide, the other probe substrate of cytochrome P450
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Center for advanced bioanalytical technologies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
36
Issue of the periodical within the volume
11-12
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
1365-1373
UT code for WoS article
000356004200017
EID of the result in the Scopus database
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