Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080814" target="_blank" >RIV/00216224:14310/15:00080814 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/15:00443283 RIV/00159816:_____/15:00062875
Result on the web
<a href="http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf" target="_blank" >http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/jacs.5b01681" target="_blank" >10.1021/jacs.5b01681</a>

Result language
angličtina
Original language name
Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.
Original language description
Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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