In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087850" target="_blank" >RIV/00216224:14310/16:00087850 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00436-015-4841-0" target="_blank" >http://dx.doi.org/10.1007/s00436-015-4841-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00436-015-4841-0" target="_blank" >10.1007/s00436-015-4841-0</a>

Result language

angličtina

Original language name

In vitro excystation of Cryptosporidium muris oocysts and viability of released sporozoites in different incubation media

Original language description

This study aimed to evaluate and document the excystation process of Cryptosporidium muris oocysts in various incubation media, and to monitor the behaviour of excysting and freshly excysted sporozoites. A test of oocyst viability, using fluorescent double staining with fluorescein diacetate and propidium iodide, was performed prior to each experimental assay. Light microscope observations confirmed that relatively often only three sporozoites were released; the fourth one either left the oocyst later together with a residual body or remained trapped within the oocyst wall. These results suggest that successful oocyst excystation is not limited by the viability of all four sporozoites. Darkening of oocysts to opaque and their specific movement (the so-called oocyst dancing) preceded the final excystation and liberation of sporozoites, while the dormant oocysts appeared refractive. The process of excystation in C.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EG - Zoology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasitology Research

ISSN

0932-0113

e-ISSN

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Volume of the periodical

115

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

1113-1121

UT code for WoS article

000370868200021

EID of the result in the Scopus database

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