Statistical comparison of excystation methods in Cryptosporidium parvum oocysts

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00468718" target="_blank" >RIV/60077344:_____/16:00468718 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/16:00088266 RIV/60076658:12220/16:43891334
Result on the web
<a href="http://dx.doi.org/10.1016/j.vetpar.2016.10.007" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2016.10.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.vetpar.2016.10.007" target="_blank" >10.1016/j.vetpar.2016.10.007</a>

Result language
angličtina
Original language name
Statistical comparison of excystation methods in Cryptosporidium parvum oocysts
Original language description
Excystation of sporozoites of Cryptosporidium parvum from oocysts is essential for successful in vitro assays. It has also been traditionally used as a measure for oocyst viability and infectivity. Laboratories use various excystation protocols so there is a need to clarify which method is the best. In this study, six different protocols for in vitro excystation of C. parvum oocysts were compared to find the most efficient excystation method (expressed as percentage excystation). Tested protocols differed in chemical pre-incubation steps, excystation media or time of incubation.nThere were significant differences in percentage of excysted oocysts among groups excysted by different methods. There were also significant differences in percentage of excysted oocysts between methods using pre-incubation with sodium hypochlorite and those without. The other variables examined; the presence of trypsin, kind of excystation medium and the incubation time, did not show statistical differences in percentage excystation among groups.nPre-incubation steps which included sodium hypochlorite, enhancing the permeability of the oocysts were found to increase the excystation ratio and methods using this step were the most effective.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EG - Zoology
OECD FORD branch
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Project
<a href="/en/project/GAP505%2F11%2F1163" target="_blank" >GAP505/11/1163: Anti-inflammatory activity of extracts isolated from selected Indonesian plants and their effect on opportunistic parasitoses</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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