All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087978" target="_blank" >RIV/00216224:14310/16:00087978 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1093/chromsci/bmw033" target="_blank" >http://dx.doi.org/10.1093/chromsci/bmw033</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1093/chromsci/bmw033" target="_blank" >10.1093/chromsci/bmw033</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).

  • Original language description

    Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC–MS-(TOF). The samples of rumen fluid and milk were obtained from 12 lactating dairy cows ingesting 7,500–9,500 mg of total isoflavones daily. The validation of the developed method showed the limits of quantification to be in the range of 0.9–5.0 ng/mL. The precision was determined as relative standard deviation, which was lower than 25% in all cases. The recoveries of the most isoflavonoids were satisfactory. Lower recoveries of daidzin and glycitin can be solved by adding an internal standard. The presented method will be useful for kinetic studies of isoflavone metabolism in ruminants due to simultaneous quantification of free aglycones and glycosides in the rumen fluid.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA13-34134S" target="_blank" >GA13-34134S: Metabolism of Isoflavones in Rumen with respect to Equol Content in Bovine Milk</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Chromatographic Science

  • ISSN

    0021-9665

  • e-ISSN

  • Volume of the periodical

    54

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

    997-1003

  • UT code for WoS article

    000385022100014

  • EID of the result in the Scopus database