Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087978" target="_blank" >RIV/00216224:14310/16:00087978 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1093/chromsci/bmw033" target="_blank" >http://dx.doi.org/10.1093/chromsci/bmw033</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/chromsci/bmw033" target="_blank" >10.1093/chromsci/bmw033</a>
Alternative languages
Result language
angličtina
Original language name
Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).
Original language description
Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC–MS-(TOF). The samples of rumen fluid and milk were obtained from 12 lactating dairy cows ingesting 7,500–9,500 mg of total isoflavones daily. The validation of the developed method showed the limits of quantification to be in the range of 0.9–5.0 ng/mL. The precision was determined as relative standard deviation, which was lower than 25% in all cases. The recoveries of the most isoflavonoids were satisfactory. Lower recoveries of daidzin and glycitin can be solved by adding an internal standard. The presented method will be useful for kinetic studies of isoflavone metabolism in ruminants due to simultaneous quantification of free aglycones and glycosides in the rumen fluid.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA13-34134S" target="_blank" >GA13-34134S: Metabolism of Isoflavones in Rumen with respect to Equol Content in Bovine Milk</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatographic Science
ISSN
0021-9665
e-ISSN
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Volume of the periodical
54
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
997-1003
UT code for WoS article
000385022100014
EID of the result in the Scopus database
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