Cystatins of helminths: characterization of molecules with immunomodulatory potential

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088207" target="_blank" >RIV/00216224:14310/16:00088207 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Cystatins of helminths: characterization of molecules with immunomodulatory potential

Original language description

Cystatins ­ cysteine peptidase inhibitors are produced by a wide range of organisms, including parasites, where they act as modulators of the host immune system. In the frame of our research we focused on characterization of cystatins produced by four helminth species representing most important groups of helminth parasites, namely Eudiplozoon nipponicum (Monogenea), Fascioloides magna (Trematoda), Hymenolepis diminuta (Cestoda) and Trichinella spiralis (Nematoda). We performed a bacterial expression of optimized cystatin genes in recombinant forms (E. coli BL21 cells, pET32a and/or pET19b plasmid vector). The recombinant cystatins were purified via His­tag using metal affinity chromatography (Ni­NTA) and successful production of cystatin was confirmed by mass spectrometric methods (MALDI TOF/TOF).

Czech name

—

Czech description

—

Type

O - Miscellaneous

CEP classification

EG - Zoology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů