Electrophoretically mediated microanalysis for simultaneous on-capillary derivatization of standard amino acids followed by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00095072" target="_blank" >RIV/00216224:14310/17:00095072 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.chroma.2017.03.080" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2017.03.080</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2017.03.080" target="_blank" >10.1016/j.chroma.2017.03.080</a>

Result language

angličtina

Original language name

Electrophoretically mediated microanalysis for simultaneous on-capillary derivatization of standard amino acids followed by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection

Original language description

Amino acids are crucial compounds involved in most biochemical processes essential for life. Since their dynamic turnover reflects the actual physiology of the cell/organism, a turnover assessment may provide valuable information related to multiple physiological and pathophysiological conditions. The sensitive determination of amino acids is predominantly associated with their derivatization which might be laborious, time-consuming and difficult to standardize. However, capillary electrophoresis offers the automatic injection and mixing of reactants, incubation of the reaction mixture, separation and detection of the reaction products in one on-capillary procedure. Among the on-capillary mixing strategies, electrophoretically mediated microanalysis (EMMA) is superior in terms of mixing efficiency. In this paper, we present an optimization of EMMA for the simultaneous derivatization of standard amino acids by naphthalene-2,3-dicarboxaldehyde/NaCN and its application to targeted human embryo metabolomics. For such a purpose, novel separation conditions were developed involving the background electrolyte, comprised of 73 mM sodium dodecyl sulfate, 6.7 % (v/v) 1-propanol, 0.5 mM (2-hydroxypropyl)-beta-cyclodextrin and 135 mM boric acid/sodium hydroxide buffer (pH 9.00). Finally, the optimized EMMA was compared to a fundamentally different mixing strategy, namely the transverse diffusion of laminar flow profiles, and proved to be also suitable for human plasma analysis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

<a href="/en/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Center for advanced bioanalytical technologies</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Volume of the periodical

1499

Issue of the periodical within the volume

May

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

203-210

UT code for WoS article

000401394200022

EID of the result in the Scopus database

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