MALDI mass spectrometry imaging at acquisition rates above 100 pixel/s

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00101298" target="_blank" >RIV/00216224:14310/18:00101298 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

MALDI mass spectrometry imaging at acquisition rates above 100 pixel/s

Original language description

The practicality of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) applied to molecular imaging of biological tissues is limited by the analysis speed. A relatively low-speed motion of xy stages is one of the factors reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging time-of-flight (TOF) mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser beam repositioning on a target plate. [1] The laser beam was focused via a modified grid into a 10 um round spot. The modified optical system of our MALDI TOF mass spectrometer allowed the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates up to ~150 pixels/s. The influence of selected parameters on the total MS imaging time was examined. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates. A single MS image comprising 100 × 100 pixels with 10 um lateral resolution was recorded in approximately 70 seconds.

Czech name

MALDI mass spectrometry imaging at acquisition rates above 100 pixel/s

Czech description

The practicality of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) applied to molecular imaging of biological tissues is limited by the analysis speed. A relatively low-speed motion of xy stages is one of the factors reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging time-of-flight (TOF) mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser beam repositioning on a target plate. [1] The laser beam was focused via a modified grid into a 10 um round spot. The modified optical system of our MALDI TOF mass spectrometer allowed the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates up to ~150 pixels/s. The influence of selected parameters on the total MS imaging time was examined. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates. A single MS image comprising 100 × 100 pixels with 10 um lateral resolution was recorded in approximately 70 seconds.

Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA15-05387S" target="_blank" >GA15-05387S: Laser desorption mass spectrometry for analysis of molecules and nanoparticles</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

CECE 2018 15th International Interdisciplinary Meeting on Bioanalysis

ISBN

9788090495951

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

19,33

Publisher name

Institute of Analytical Chemistry of the CAS, v. v. i.

Place of publication

Brno, Czech Republic

Event location

Brno

Event date

Jan 1, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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