MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00107314" target="_blank" >RIV/00216224:14310/19:00107314 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/19:00071020 RIV/00216305:26210/19:PU135851
Result on the web
<a href="https://link.springer.com/article/10.1007/s13361-018-2078-8" target="_blank" >https://link.springer.com/article/10.1007/s13361-018-2078-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s13361-018-2078-8" target="_blank" >10.1007/s13361-018-2078-8</a>

Result language
angličtina
Original language name
MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second
Original language description
The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-m round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100x100pixels with 10-m lateral resolution was recorded in approximately 70s.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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