Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00112613" target="_blank" >RIV/00216224:14310/18:00112613 - isvavai.cz</a>

Result on the web

<a href="http://nopr.niscair.res.in/bitstream/123456789/43663/1/IJEB%2056%283%29%20180-193.pdf" target="_blank" >http://nopr.niscair.res.in/bitstream/123456789/43663/1/IJEB%2056%283%29%20180-193.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek

Original language description

Vigna radiata (L.) R.Wilczek (Fabaceae), commonly called Green gram or Mung bean, is an important legume with potential nutritional, medicinal and health benefits cultivated widespread throughout the rain-fed areas of arid and semi-arid tropics and subtropics. Being an affordable source of carbohydrate, vitamins, minerals and phytonutrients besides protein, Green gram finds demand for its nutrient digestibility, food processing properties and bioavailability. Though India ranks top in world mung bean production (&gt;50%), it is unable to meet the local demand. Biotic and abiotic stresses restrict mung bean yield considerably and researchers have been working on resistant varieties to overcome these challenges. In this study, towards improving yield, an effective protocol for attaining high frequency somatic embryogenesis (SE) in green gram has been proposed. Type of explants and age of source seedlings for obtaining explants were found to influence the formation of embryogenic calli. Various combinations and concentrations of 2,4-dichlorophenoxyacetic acid and indole-3-acetic acid with kinetin were optimized for developing embryogenic calli. Embryogenic calli when exposed to osmotic stress created by D-mannitol and sorbitol and dehydration stress imposed by polyethylene glycol were found to produce somatic embryos. Calli incubated for 6 h in specified hormone free nutrient medium supplemented with 4% polyethylene glycol was optimal for induction of high frequency SE. Subsequent to stress incubation, the cultures formed only early stage somatic embryos. Supplementation of proline was found essential for the maturation of somatic embryos. Cotyledonary stage somatic embryos were converted into plantlets and subsequently established in garden soil. Semi-quantitative Reverse Transcription-PCR based transcript level analysis of SERK1 gene expression was carried out during different developmental stages of somatic embryogenesis. Expression of SERK1 was specifically associated with the embryogenic calli and calli enriched with globular stage somatic embryos.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY

ISSN

0019-5189

e-ISSN

0975-1009

Volume of the periodical

56

Issue of the periodical within the volume

3

Country of publishing house

IN - INDIA

Number of pages

14

Pages from-to

180-193

UT code for WoS article

000427449400005

EID of the result in the Scopus database

2-s2.0-85045345292