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Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F20%3A00114288" target="_blank" >RIV/00216224:14310/20:00114288 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1007/978-1-0716-0763-3_11" target="_blank" >https://doi.org/10.1007/978-1-0716-0763-3_11</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-0716-0763-3_11" target="_blank" >10.1007/978-1-0716-0763-3_11</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres

  • Original language description

    Characterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. Here we describe optimized methods and their mutual combinations for this purpose: bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP), yeast two-hybrid systems (Y2H), and chromatin immunoprecipitation (ChIP). These improved protocols detect trimeric complexes in which two proteins of interest interact indirectly via a protein sandwiched between them. They also allow isolation of low-abundance chromatin proteins and confirmation that proteins of interest are associated with specific DNA sequences, for example telomeric tracts. Here we describe these methods and their application to map interactions of several telomere- and telomerase-associated proteins and to purify a sufficient amount of chromatin from Arabidopsis thaliana for further investigations (e.g., next-generation sequencing, hybridization)

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    The Nucleus - Methods in Molecular Biology

  • ISBN

    9781071607626

  • Number of pages of the result

    29

  • Pages from-to

    139-167

  • Number of pages of the book

    220

  • Publisher name

    Springer Nature, Humana Press

  • Place of publication

    New York, USA

  • UT code for WoS chapter

    000681366300012