Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00604833" target="_blank" >RIV/68081707:_____/20:00604833 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-0763-3_11" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-0763-3_11</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-0763-3_11" target="_blank" >10.1007/978-1-0716-0763-3_11</a>
Alternative languages
Result language
angličtina
Original language name
Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres
Original language description
Characterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. Here we describe optimized methods and their mutual combinations for this purpose: bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP), yeast two-hybrid systems (Y2H), and chromatin immunoprecipitation (ChIP). These improved protocols detect trimeric complexes in which two proteins of interest interact indirectly via a protein sandwiched between them. They also allow isolation of low-abundance chromatin proteins and confirmation that proteins of interest are associated with specific DNA sequences, for example telomeric tracts. Here we describe these methods and their application to map interactions of several telomere- and telomerase-associated proteins and to purify a sufficient amount of chromatin from Arabidopsis thaliana for further investigations (e.g., next-generation sequencing, hybridization).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods in Molecular Biology
ISSN
1064-3745
e-ISSN
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Volume of the periodical
2175
Issue of the periodical within the volume
2021-08-12
Country of publishing house
US - UNITED STATES
Number of pages
29
Pages from-to
139-167
UT code for WoS article
000681366300012
EID of the result in the Scopus database
2-s2.0-85088227850