Transmembrane Transport of Bicarbonate Unravelled**
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00119180" target="_blank" >RIV/00216224:14310/21:00119180 - isvavai.cz</a>
Result on the web
<a href="https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/chem.202100491" target="_blank" >https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/chem.202100491</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/chem.202100491" target="_blank" >10.1002/chem.202100491</a>
Alternative languages
Result language
angličtina
Original language name
Transmembrane Transport of Bicarbonate Unravelled**
Original language description
Anion receptors can be used to transport ions across lipid bilayers, which has potential for therapeutic applications. Synthetic bicarbonate transporters are of particular interest, as defects in transmembrane transport of bicarbonate are associated with various diseases. However, no convenient method exists to directly observe bicarbonate transport and study the mechanisms involved. Here, an assay is presented that allows the kinetics of bicarbonate transport into liposomes to be monitored directly and with great sensitivity. The assay utilises an encapsulated europium(III) complex, which exhibits a large increase in emission intensity upon binding bicarbonate. Mechanisms involving CO2 diffusion and the dissipation of a pH gradient are shown to be able to lead to an increase in bicarbonate concentration within liposomes, without transport of the anion occurring at all. By distinguishing these alternative mechanisms from actual bicarbonate transport, this assay will inform the future development of bicarbonate transporters.
Czech name
—
Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10400 - Chemical sciences
Result continuities
Project
<a href="/en/project/GA20-13922S" target="_blank" >GA20-13922S: Monofunctionalized bambusurils and their supramolecular properties</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemistry - A European Journal
ISSN
0947-6539
e-ISSN
—
Volume of the periodical
27
Issue of the periodical within the volume
26
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
7367-7375
UT code for WoS article
000645925400001
EID of the result in the Scopus database
2-s2.0-85105089826