The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00135421" target="_blank" >RIV/00216224:14310/24:00135421 - isvavai.cz</a>
Result on the web
<a href="https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/anse.202300044" target="_blank" >https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/anse.202300044</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/anse.202300044" target="_blank" >10.1002/anse.202300044</a>
Alternative languages
Result language
angličtina
Original language name
The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles
Original language description
Synthetic anion transporters are developed to transport anions across lipid membranes with the long-term perspective of biological applications. The lucigenin assay is a popular tool to study their transport of chloride and other anions in liposomes. It relies on the quenching of the fluorescence of encapsulated lucigenin by anions, which can be monitored by fluorescence spectroscopy. This article provides a tutorial introduction to the practical use and understanding of the lucigenin assay. It describes in detail how to use this assay to monitor chloride/nitrate antiport in liposomes, process and interpret the data, and solve common issues. Variations of the assay enabling the investigation of the transport of other anions and transport mechanisms are discussed. Furthermore, a zwitterionic analogue of lucigenin is demonstrated to have advantages for use in experiments over longer time scales, as it does not leak out of the liposomes, or when studying chloride uniport, as it avoids interference from antiport with nitrate that is present in commercial lucigenin.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analysis and Sensing
ISSN
2629-2742
e-ISSN
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Volume of the periodical
4
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
16
Pages from-to
1-16
UT code for WoS article
001119196500001
EID of the result in the Scopus database
2-s2.0-85170680023