DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00121537" target="_blank" >RIV/00216224:14310/21:00121537 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/21:PU140094
Result on the web
<a href="https://elifesciences.org/articles/60916#xb0ffa41b" target="_blank" >https://elifesciences.org/articles/60916#xb0ffa41b</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7554/eLife.60916" target="_blank" >10.7554/eLife.60916</a>
Alternative languages
Result language
angličtina
Original language name
DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome
Original language description
Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1(Ndr/Ndr) mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1(Ndr/Ndr) liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
eLife
ISSN
2050-084X
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
February
Country of publishing house
GB - UNITED KINGDOM
Number of pages
29
Pages from-to
1-29
UT code for WoS article
000625357100001
EID of the result in the Scopus database
2-s2.0-85101937944