DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU141859" target="_blank" >RIV/00216305:26620/21:PU141859 - isvavai.cz</a>
Result on the web
<a href="https://www.jove.com/t/62941/duct-double-resin-casting-followed-micro-computed-tomography-for-3d" target="_blank" >https://www.jove.com/t/62941/duct-double-resin-casting-followed-micro-computed-tomography-for-3d</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/62941" target="_blank" >10.3791/62941</a>
Alternative languages
Result language
angličtina
Original language name
DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis
Original language description
The liver is the biggest internal organ in humans and mice, and high auto-fluorescence presents a significant challenge for assessing the three-dimensional (3D) architecture of the organ at the whole-organ level. Liver architecture is characterized by multiple branching lumenized structures, which can be filled with resin, including vascular and biliary trees, establishing a highly stereotyped pattern in the otherwise hepatocyte-rich parenchyma. This protocol describes the pipeline for performing double resin casting micro-computed tomography, or "DUCT". DUCT entails injecting the portal vein and common bile duct with two different radiopaque synthetic resins, followed by tissue fixation. Quality control by clearing one lobe, or the entire liver, with an optical clearing agent, allows for pre-screening of suitably injected samples. In the second part of the DUCT pipeline, a lobe or the whole liver can be used for micro-computed tomography (microCT) scanning, (semi-)automated segmentation, and 3D rendering of the portal venous and biliary networks. MicroCT results in 3D coordinate data for the two resins allowing for qualitative as well as quantitative analysis of the two systems and their spatial relationship. DUCT can be applied to postnatal and adult mouse liver and can be further extended to other tubular networks, for example, vascular networks and airways in the lungs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30502 - Other medical science
Result continuities
Project
<a href="/en/project/LM2018110" target="_blank" >LM2018110: CzechNanoLab research infrastructure</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of visualized experiments : JoVE
ISSN
1940-087X
e-ISSN
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Volume of the periodical
175
Issue of the periodical within the volume
e62941
Country of publishing house
US - UNITED STATES
Number of pages
19
Pages from-to
1-19
UT code for WoS article
000708095700039
EID of the result in the Scopus database
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