TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN SILENE LATIFOLIA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00131845" target="_blank" >RIV/00216224:14310/23:00131845 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
TOOLS TO STUDY DOSAGE COMPENSATION AND PARENTAL IMPRINTING IN SILENE LATIFOLIA
Original language description
Dosage compensatory mechanism (DC) refers to the equalization of X-linked gene products between males and females. In old sex chromosomes systems, DC usually affects the entire X chromosomes with only few genes capable of inactivation escape. Nevertheless, in species with evolutionary young sex chromosomes, DC exhibits rather local or incomplete X chromosome inactivation, proceeding gene-by-gene or by whole locus deregulation. Despite the evidence given for the main animal species, the mechanisms through X chromosome inactivation evolves remain still enigmatic. To study early stages of DC and expression balance between sexes, we use combination of cytogenetic and NGS techniques in a species with young sex chromosome system, Silene latifolia. Using X chromosome specific marker and EdU that is incorporated into newly synthesized DNA strands, we distinguished paternal and maternal Xs. We show that parental X chromosome in females is late replicated, supported by previous evidence of transcriptome profiling and localization of active histone marks. Interestingly, only a chromosomal arm carrying PAR shows replication delay that suggests locus specific incomplete DC. Further, we ask weather polyploidy may disturb dosage compensation pattern in this model plant. To answer this question, we developed a set of tetraploid and triploid individuals with different sex chromosome constitution. This will help us to understand how and what (epi)genetic processes take a role in DC evolution, and help us to shed more light on the most fundamental question of sex chromosome biology.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů