Single particle inductively coupled plasma mass spectrometry imaging of immunochemically labeled spheroid sections

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00134084" target="_blank" >RIV/00216224:14310/23:00134084 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Single particle inductively coupled plasma mass spectrometry imaging of immunochemically labeled spheroid sections
Original language description
Spheroids are one of the 3D biological models in which the arrangement of proliferating and necrotic cells resembles the tumor microenvironment. One of the markers of proliferation is Ki-67 protein, which is expressed by cells in the active phase of the cell cycle. Immunohistochemical labeling with a primary antibody and a fluorophore-labeled secondary antibody followed by fluorescence microscopy analysis is commonly employed for two- and three-dimensional imaging of the target protein in spheroid sections. We have successfully switched from fluorophore labeling of Ki-67 to nanoparticle labeling combined with LA ICP MS.* Recently, gold nanoparticles were used as very sensitive immunochemical tags in conventional UV laser ablation ICP MSI.1,2 In our approach, intact 20 nm gold nanoparticles tags were desorbed from the spheroid section with 2 940 nm laser ablation system.
Czech name
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Czech description
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Project
<a href="/en/project/GA21-12262S" target="_blank" >GA21-12262S: Nanoparticle probes for mass spectrometry imaging</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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