Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00137748" target="_blank" >RIV/00216224:14310/24:00137748 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-024-72169-6" target="_blank" >https://www.nature.com/articles/s41598-024-72169-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-024-72169-6" target="_blank" >10.1038/s41598-024-72169-6</a>
Alternative languages
Result language
angličtina
Original language name
Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesis
Original language description
This study introduces a novel cost-effective technique for cloning of linear DNA plasmid inserts, aiming to address the associated expenses linked with popular in vitro DNA assembly methods. Specifically, we introduce ECOLI (Efficient Cloning Of Linear Inserts), a method utilizing a PCR product-based site-directed mutagenesis. In comparison to other established in vitro DNA assembly methods, our approach is without the need for costly synthesis or specialized kits for recombination or restriction sites. ECOLI offers a fast, efficient, and economical alternative for cloning inserts up to several hundred nucleotides into plasmid constructs, thus enhancing cloning accessibility and efficiency. This method can enhance molecular biology research, as we briefly demonstrated on the Dishevelled gene from the WNT signaling pathway.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10605 - Developmental biology
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
—
Volume of the periodical
14
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
1-8
UT code for WoS article
001337075200038
EID of the result in the Scopus database
2-s2.0-85204172292