Efficient Mutagenesis Independent of Ligation (EMILI)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897505" target="_blank" >RIV/60461373:22330/14:43897505 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0167701214002371" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0167701214002371</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mimet.2014.08.003" target="_blank" >10.1016/j.mimet.2014.08.003</a>
Alternative languages
Result language
angličtina
Original language name
Efficient Mutagenesis Independent of Ligation (EMILI)
Original language description
Site-directed mutagenesis is one of the most widely used techniques in life sciences. Here we describe an improved and simplified method for introducing mutations at desired sites. It consists of an inverse PCR using a plasmid template and two partiallycomplementary primers. The synthesis step is followed by annealing of the PCR product's sticky ends, which are generated by exonuclease digestion. This method is fast, extremely efficient and cost-effective. It can be used to introduce large insertions and deletions, but also for multiple point mutations in a single step. To show the principle and to prove the efficiency of the method, we present a series of basic mutations (insertions, deletions, point mutations) on pUC19 plasmid DNA.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP302%2F12%2F1895" target="_blank" >GAP302/12/1895: Role of retroviral matrix protein in virus particle targeting and interaction with plasma membrane.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Microbiological Methods
ISSN
0167-7012
e-ISSN
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Volume of the periodical
106
Issue of the periodical within the volume
listopad 2014
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
5
Pages from-to
67-71
UT code for WoS article
000343358200011
EID of the result in the Scopus database
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