Immunomagnetic separation in the detection of Salmonella cells from foodstuffs

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F99%3A00004086" target="_blank" >RIV/00216224:14310/99:00004086 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Immunomagnetic separation in the detection of Salmonella cells from foodstuffs
Original language description
Cultivation method and polymerase chain reaction coupled with immunomagnetic separation (IMS-CM and IMS-PCR) are new technoques used for the detection of bacteria in foodstuffs. In this study, Salmonella was isolated from various real food samples by magnetic Dynabeads M-280 anti-Salmonella using both IMS-CM and IMS-PCR. Food samples included (milk, eggs, meat products, confectionery, vegetable, fruit products) and total 408 specimens were investigated. 49 samples (i.e. 12%) were found Salmonella positive by IMS-CM. Bacteria coupled on immunomagnetic particles were also used in IMS-PCR. Growing atypically on sensitive medium two Salmonella strains were identified using IMS-PCR. False negative results were obtained by conbventional technques. The abovementioned results demonstrated that IMS-CM and IMS-PCR techniques are more sensetive in real food samples analysis than conventional cultivation procedures.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
1999
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
13th Internal Symposium of Affinity Technology and Bio-Recognition
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
68
Publisher name
Université de Technologie de Compiegne
Place of publication
Compiegne
Event location
Compiegne
Event date
Jan 1, 1999
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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