Distinct patterns of histone methylation and acetylation in human interphase nuclei

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F07%3A00020185" target="_blank" >RIV/00216224:14330/07:00020185 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/07:00314104
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Distinct patterns of histone methylation and acetylation in human interphase nuclei
Original language description
To study 3D nuclear distributions of epigenetic histone modifications such as H3(K9) acetylation, H3(K4) dimethylation, H3(K9) dimethylation, and H3(K27) trimethylation, and of histone methyl transferase Suv39H1, we used advanced image analysis methods,combined with Nipkow disk confocal microscopy. Total fluorescence intensity and distributions of fluorescently labelled proteins were analysed in formaldehyde-fixed interphase nuclei. Our data showed reduced fluorescent signals of H3(K9) acetylation andH3(K4) dimethylation (dime) at the nuclear periphery, while di-meH3(K9) was also abundant in chromatin regions closely associated with the nuclear envelope. Little overlapping (intermingling) was observed for di-meH3(K4) and H3(K27) trimethylation (tri-me), and for di-meH3(K9) and Suv39H1. The histone modifications studied were absent in the nucleolar compartment with an exception of H3(K9) dimethylation that was closely associated to perinucleolar regions which are formed by centromeres
Czech name
Rozdílné paterny metylace a acetylace histonů v lidských interfázních jádrech
Czech description
Práce se zabývá různými "paterny" metylace a acetylace histonů v lidských interfázních jádrech

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)