Performance and Sensitivity Evaluation of 3-D Spot Detection Methods in Confocal Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F15%3A00080577" target="_blank" >RIV/00216224:14330/15:00080577 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/cyto.a.22692" target="_blank" >http://dx.doi.org/10.1002/cyto.a.22692</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.22692" target="_blank" >10.1002/cyto.a.22692</a>
Alternative languages
Result language
angličtina
Original language name
Performance and Sensitivity Evaluation of 3-D Spot Detection Methods in Confocal Microscopy
Original language description
Reliable 3D detection of diffraction-limited spots in fluorescence microscopy images is an important task in subcellular observation. Generally, fluorescence microscopy images are heavily degraded by noise and non-specifically stained background, makingreliable detection a challenging task. In this work, we have studied the performance and parameter sensitivity of eight recent methods for 3D spot detection. The study is based on both 3D synthetic image data and 3D real confocal microscopy images. The synthetic images were generated using a simulator modeling the complete imaging setup, including the optical path as well as the image acquisition process. We studied the detection performance and parameter sensitivity under different noise levels and under the influence of uneven background signal. To evaluate the parameter sensitivity, we propose a novel measure based on the gradient magnitude of the F1 score.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
IN - Informatics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytometry Part A
ISSN
1552-4922
e-ISSN
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Volume of the periodical
87A
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
759-772
UT code for WoS article
000358643200011
EID of the result in the Scopus database
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