Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F11%3A00052199" target="_blank" >RIV/00216224:14740/11:00052199 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.syapm.2010.11.008" target="_blank" >http://dx.doi.org/10.1016/j.syapm.2010.11.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.syapm.2010.11.008" target="_blank" >10.1016/j.syapm.2010.11.008</a>

Result language

angličtina

Original language name

Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species

Original language description

The influence of the matrix solution, sample form and deposition technique on the quality MALDI-TOF mass spectra was examined and assessed with the aim to improve MALDI-TOF MS performance for the identification of microorganisms and to enable automatic spectra acquisition. It was observed that the use of matrix compounds ferulic and sinapinic acid may result in improved mass spectral features, in terms of signal resolution and S/N ratio, as compared to alpha-cyano-4-hydroxycinnamic acid, which was, on the other hand, found to be the only matrix compound that enabled fully automatic mass spectra acquisition. The robustness of the whole sample preparation procedure was then assessed on a set of 25 strains of four Acinetobacter species. Results showed reproducible detection of subtle mass spectral differences between strains belonging to the same species, although they do not confirm the possibility of reliable strain typing.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Systematic and Applied Microbiology

ISSN

0723-2020

e-ISSN

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Volume of the periodical

34

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

30-34

UT code for WoS article

000289395200005

EID of the result in the Scopus database

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