Spektroskopická charakterizace aptasenzoru specifického pro thrombin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F12%3A00058010" target="_blank" >RIV/00216224:14740/12:00058010 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Spektroskopická charakterizace aptasenzoru specifického pro thrombin
Original language description
Gold nanoparticles (GNPs) represent a very attractive material for the construction of sensors and biosensors with immobilized aptamer on the surface, due to their biocompatibility. The most extensively studied aptamer is the thrombin binding aptamer (TBA) with sequence GGT TGG TGT GGT TGG, specifically binding to thrombin, and thus inhibits the activity of thrombin in the cascade of reactions resulting in the hemocoagulation. The aim of our contribution is spectroscopic characterization of aptasensor system and verify its specificity to thrombin in buffered and non ? buffered aqueous solutions with adjusted ionic strength. For our experiment the GNPs (5, 10 and 20nm) stabilized in citrate buffer were used. The absorption spectra were monitored not only in the dependence on particles size, but also on the components of used solutions. For the detection of aggregation and behavior of unmodified and modified GNPs the distribution of GNPs and their zeta potential were determined.
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GD106%2F09%2FH035" target="_blank" >GD106/09/H035: Multiscale design of advanced materials</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Víceúrovňový design pokrokových materiálů, sborník doktorské konference 2012
ISBN
9788087434062
ISSN
—
e-ISSN
—
Number of pages
8
Pages from-to
147-154
Publisher name
Ústav fyziky materiálů AV ČR, v. v. i.
Place of publication
Brno
Event location
Brno
Event date
Jan 1, 2012
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
—