The structure and substrate specificity of human Cdk12/Cyclin K
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073691" target="_blank" >RIV/00216224:14740/14:00073691 - isvavai.cz</a>
Result on the web
<a href="http://www.nature.com/ncomms/2014/140324/ncomms4505/pdf/ncomms4505.pdf" target="_blank" >http://www.nature.com/ncomms/2014/140324/ncomms4505/pdf/ncomms4505.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/ncomms4505" target="_blank" >10.1038/ncomms4505</a>
Alternative languages
Result language
angličtina
Original language name
The structure and substrate specificity of human Cdk12/Cyclin K
Original language description
Phosphorylation of the RNA polymerase II C-terminal domain (CTD) by cyclin-dependent kinases is important for productive transcription. Here we determine the crystal structure of Cdk12/CycK and analyse its requirements for substrate recognition. Active Cdk12/CycK is arranged in an open conformation similar to that of Cdk9/CycT but different from those of cell cycle kinases. Cdk12 contains a C-terminal extension that folds onto the N- and C-terminal lobes thereby contacting the ATP ribose. The interaction is mediated by an HE motif followed by a polybasic cluster that is conserved in transcriptional CDKs. Cdk12/CycK showed the highest activity on a CTD substrate prephosphorylated at position Ser7, whereas the common Lys7 substitution was not recognized.Flavopiridol is most potent towards Cdk12 but was still 10-fold more potent towards Cdk9. T-loop phosphorylation of Cdk12 required coexpression with a Cdk-activating kinase.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
NATURE COMMUNICATIONS
ISSN
2041-1723
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
3505
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
"nestránkováno"
UT code for WoS article
000334302000008
EID of the result in the Scopus database
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