Stability of human telomere quadruplexes at high DNA concentrations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00074985" target="_blank" >RIV/00216224:14740/14:00074985 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/14:00435395

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/bip.22400" target="_blank" >10.1002/bip.22400</a>

Result language

angličtina

Original language name

Stability of human telomere quadruplexes at high DNA concentrations

Original language description

For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A different phenomenon was observed, and is presented in this article, when the crowder was the quadruplex itself. With DNA strand concentrations ranging from 3 mM to 9 mM, the thermostabilitydid not change up to 2 mM, above which it increased, indicating that the unfolding quadruplex units were not monomolecular above 2 mM. The results are explained by self-association of the G-quadruplexes above this concentration. The deltaG(o) 37 values,evaluated only below 2 mM, did not become more negative, as with the non-DNA crowders, instead, slightly increased. Folding topology changed from antiparallel to hybrid above 2 mM, and then to parallel quadruplexes at high, 6-9 mM strand

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biopolymers

ISSN

0006-3525

e-ISSN

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Volume of the periodical

101

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

428-438

UT code for WoS article

000336575300013

EID of the result in the Scopus database

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