Formation of G-quadruplex and Its Utilizing for an Automated Spectrometric Detection of Cisplatin

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43907681" target="_blank" >RIV/62156489:43210/15:43907681 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/15:PU118485

Result on the web

<a href="http://www.electrochemsci.org/papers/vol10/100503973.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol10/100503973.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Formation of G-quadruplex and Its Utilizing for an Automated Spectrometric Detection of Cisplatin

Original language description

Here, we studied the formation of G-quadruplex using spectrophotometric methods (peroxidase activity of G-quadruplex) and verifications of obtained results by voltammetry (cytosine-adenine (CA) or guanine (G) peaks height). The results showed that the peroxidase activity of G-quadruplex increases with the increasing concentrations of K+ ions (1-20 mM) and Na+ ions (1-5 mM) with the highest values observed in the presence of K+ ions. The reverse trend was obtained with NH4+ ions, where the highest peroxidase activity was observed at the lowest concentration of NH4+ ions. The G-quadruplex formation was also proven electrochemically. The addition of 5 mM of K+ ions to the oligonucleotide solution with hemin caused a decrease of both determined peaks. Theperoxidase activity of G-quadruplex increased with the increasing concentration of Ag+ ions within the range from 40 nM to 5 mu M. Further, the addition of different concentration of cisplatin (0.5-2 mu M) to the G-quadruplex was monitore

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GAP102%2F11%2F1068" target="_blank" >GAP102/11/1068: Nano-Electro-Bio-Tools for Biochemical and Molecularly-Biological Studies of Eukaryotic Cells (NanoBioTECell)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

5

Country of publishing house

CS - SERBIA AND MONTENEGRO

Number of pages

15

Pages from-to

3973-3987

UT code for WoS article

000354782200024

EID of the result in the Scopus database

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