DNA POLYMERASE STOP ASSAY FOR DETECTION OF G-QUADRUPLEXES

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00506727" target="_blank" >RIV/68081707:_____/14:00506727 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
DNA POLYMERASE STOP ASSAY FOR DETECTION OF G-QUADRUPLEXES
Original language description
In this work we performed DNA polymerase stop assays while using template DNA derived from promoter regions of VEGF and c-Myc proto-oncogenes under variety of experimental conditions. Partial stopping of DNA synthesis along the template strand was observed in the presence of K+ ions due to formation of stable G-quadruplex structures. In contrast, Na+ ions alone were unable to stabilize G-quadruplexes to stop the reaction at their site. These data suggest that K+ and Na+ ions, which are both known to stabilize G-quadruplexes, do this in different manner or extent.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis
ISBN
978-80-904959-2-0
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
"Č. 2014 (2014)"
Publisher name
Institute of Analytical Chemistry AS CR
Place of publication
Brno
Event location
Brno
Event date
Oct 20, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000354547400114

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