Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00079244" target="_blank" >RIV/00216224:14740/14:00079244 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.4049/jimmunol.1302064" target="_blank" >http://dx.doi.org/10.4049/jimmunol.1302064</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4049/jimmunol.1302064" target="_blank" >10.4049/jimmunol.1302064</a>

Result language
angličtina
Original language name
Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling
Original language description
The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y. We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly linearly with age, with significant reduction already apparent by age 40. The percentage of naive T cells showed a strong correlation with measured TCR diversity and decreased linearly up to age 70. Remarkably, the oldest group (average age 82 y) was characterized by a higher percentage of naive CD4(+) T cells, lower abundance of expanded clones, and increased TCR diversity compared with the previous age group (average age 62 y), suggesting the influence of age selection and association of these three related parameters with longevity.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FB - Endocrinology, diabetology, metabolism, nutrition
OECD FORD branch
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Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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