Tracking T-cell immune reconstitution after TCR alpha beta/CD19-depleted hematopoietic cells transplantation in children

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00100338" target="_blank" >RIV/00216224:14740/17:00100338 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/leu2016321.pdf" target="_blank" >https://www.nature.com/articles/leu2016321.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/leu.2016.321" target="_blank" >10.1038/leu.2016.321</a>

Result language

angličtina

Original language name

Tracking T-cell immune reconstitution after TCR alpha beta/CD19-depleted hematopoietic cells transplantation in children

Original language description

alpha beta T-cell-depleted allogeneic hematopoietic cell transplantation holds promise for the safe and accessible therapy of both malignant and non-malignant blood disorders. Here we employed molecular barcoding normalized T-cell receptor (TCR) profiling to quantitatively track T-cell immune reconstitution after TCR alpha beta-/CD19-depleted transplantation in children. We demonstrate that seemingly early reconstitution of alpha beta T-cell counts 2 months after transplantation is based on only several hundred rapidly expanded clones originating from non-depleted graft cells. In further months, frequency of these hyperexpanded clones declines, and after 1 year the observed T-cell counts and TCR beta diversity are mostly provided by the newly produced T cells. We also demonstrate that high TCR beta diversity at day 60 observed for some of the patients is determined by recipient T cells and intrathymic progenitors that survived conditioning regimen. Our results indicate that further efforts on optimization of TCR alpha beta-/CD19-depleted transplantation protocols should be directed toward providing more efficient T-cell defense in the first months after transplantation.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Leukemia

ISSN

0887-6924

e-ISSN

—

Volume of the periodical

31

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

1145-1153

UT code for WoS article

000400464800013

EID of the result in the Scopus database

2-s2.0-85001832885