Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00079627" target="_blank" >RIV/00216224:14740/14:00079627 - isvavai.cz</a>

Result on the web

<a href="http://pubs.acs.org/doi/abs/10.1021/am500732y" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/am500732y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/am500732y" target="_blank" >10.1021/am500732y</a>

Result language

angličtina

Original language name

Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates

Original language description

Photon-upconverting nanoparticles (UCNPs) have attracted much interest as a new class of luminescent label for the background-free detection in bioanalytical applications. UCNPs and other nanoparticles are commonly coated with a silica shell to improve their dispersibility and chemical stability in aqueous buffer and to incorporate functional groups for subsequent bioconjugation steps. The process of silica coating, however, is difficult to control without suitable analytical and preparative methods. Here, we have introduced agarose gel electrophoresis for the analysis and purification of silica-coated UCNPs. The silica shell can be doped with a fluorescent dye for direct detection in the gel without influencing the structure or electrophoretic mobility of the nanoparticles. The preparation of a bare silica shell by reverse microemulsion resulted in individual nanoparticles but also distinct aggregates that could be separated and isolated from the agarose gel.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ACS APPLIED MATERIALS &amp; INTERFACES

ISSN

1944-8244

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

6930-6935

UT code for WoS article

000336075300111

EID of the result in the Scopus database

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