Large-scale purification of photon-upconversion nanoparticles by gel electrophoresis for analogue and digital bioassays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F19%3A00504889" target="_blank" >RIV/68081715:_____/19:00504889 - isvavai.cz</a>
Alternative codes found
RIV/68081723:_____/19:00504889
Result on the web
<a href="http://hdl.handle.net/11104/0296431" target="_blank" >http://hdl.handle.net/11104/0296431</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.8b04488" target="_blank" >10.1021/acs.analchem.8b04488</a>
Alternative languages
Result language
angličtina
Original language name
Large-scale purification of photon-upconversion nanoparticles by gel electrophoresis for analogue and digital bioassays
Original language description
The performance of photon-upconversion nanoparticles (UCNPs) as background-free luminescent labels in bioanalytical applications strongly depends on the preparation of well-defined and water-dispersible nanoconjugates. We have exploited the separation power of agarose-gel electrophoresis to purify milligram amounts of homogeneous UCNPs covered with carboxylated silica, biotin, or streptavidin with recovery rates of 30 to 50%. Clusters containing discrete numbers of UCNPs were isolated from the gel and reanalyzed by agarose-gel electrophoresis, single-nanoparticle-upconversion microscopy, and additional complementary methods. The purified nanoconjugates improved conventional (analogue) bioaffinity assays and provided highly monodisperse conjugates for assays that rely on counting individual UCNPs (digital assays).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GJ18-03367Y" target="_blank" >GJ18-03367Y: Photon upconversion barcoding for bioanalysis in droplet microfluidics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
—
Volume of the periodical
91
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
1241-1246
UT code for WoS article
000456350000007
EID of the result in the Scopus database
2-s2.0-85059606468