Automated Microscopy in Forward Genetic Screening of Arabidopsis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00080711" target="_blank" >RIV/00216224:14740/14:00080711 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4" target="_blank" >http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-62703-643-6_4" target="_blank" >10.1007/978-1-62703-643-6_4</a>

Result language
angličtina
Original language name
Automated Microscopy in Forward Genetic Screening of Arabidopsis
Original language description
Tightly controlled spatiotemporal specificity of gene expression is intrinsic to developmental and adaptation responses of living systems throughout the kingdoms. Forward genetic screens employing well-characterized reporter lines can be used to identifyas yet unknown genetic factors driving specific aspects of individual regulatory pathways. However, such screens are demanding with respect to data acquisition and analysis from thousands of mutant lines. Here, we describe a method that allows screeningof a mutagenized GUS reporter line in Arabidopsis using an automated microscopy imaging system as a tool for rapid and efficient identification of mutants with modified expression profile for a gene of interest.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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