Post-Translational Modifications of Histones in Human Sperm
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00082822" target="_blank" >RIV/00216224:14740/15:00082822 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/15:00447571
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jcb.25170/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jcb.25170/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcb.25170" target="_blank" >10.1002/jcb.25170</a>
Alternative languages
Result language
angličtina
Original language name
Post-Translational Modifications of Histones in Human Sperm
Original language description
We examined the levels and distribution of post-translationally modified histones and protamines in human sperm. Using western blot immunoassay, immunofluorescence, mass spectrometry (MS), and FLIM-FRET approaches, we analyzed the status of histone modifications and the protamine P2. Among individual samples, we observed variability in the levels of H3K9me1, H3K9me2, H3K27me3, H3K36me3, and H3K79me1, but the level of acetylated (ac) histones H4 was relatively stable in the sperm head fractions, as demonstrated by western blot analysis. Sperm heads with lower levels of P2 exhibited lower levels of H3K9ac, H3K9me1, H3K27me3, H3K36me3, and H3K79me1. A very strong correlation was observed between the levels of P2 and H3K9me2. FLIM-FRET analysis additionally revealed that acetylated histones H4 are not only parts of sperm chromatin but also appear in a non-integrated form. Intriguingly, H4ac and H3K27me3 were detected in sperm tail fractions via western blot analysis.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of cellular biochemistry
ISSN
0730-2312
e-ISSN
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Volume of the periodical
116
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
2195-2209
UT code for WoS article
000359809400009
EID of the result in the Scopus database
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