Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00085377" target="_blank" >RIV/00216224:14740/15:00085377 - isvavai.cz</a>

Result on the web

<a href="http://ac.els-cdn.com/S2211124715007056/1-s2.0-S2211124715007056-main.pdf?_tid=b2a09f7c-a955-11e5-8086-00000aab0f6c&acdnat=1450862306_2c1bb6f674674ff62a019a857bbab0ea" target="_blank" >http://ac.els-cdn.com/S2211124715007056/1-s2.0-S2211124715007056-main.pdf?_tid=b2a09f7c-a955-11e5-8086-00000aab0f6c&acdnat=1450862306_2c1bb6f674674ff62a019a857bbab0ea</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.celrep.2015.06.067" target="_blank" >10.1016/j.celrep.2015.06.067</a>

Result language

angličtina

Original language name

Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells

Original language description

In mammals, the noncoding Xist RNA triggers transcriptional silencing of one of the two X chromosomes in female cells. Here, we report a genetic screen for silencing factors in X chromosome inactivation using haploid mouse embryonic stem cells (ESCs) that carry an engineered selectable reporter system. This system was able to identify several candidate factors that are genetically required for chromosomal repression by Xist. Among the list of candidates, we identify the RNA-binding protein Spen, the homolog of split ends. Independent validation through gene deletion in ESCs confirms that Spen is required for gene repression by Xist. However, Spen is not required for Xist RNA localization and the recruitment of chromatin modifications, including Polycomb protein Ezh2. The identification of Spen opens avenues for further investigation into the gene-silencing pathway of Xist and shows the usefulness of haploid ESCs for genetic screening of epigenetic pathways.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Reports

ISSN

2211-1247

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

554-561

UT code for WoS article

000358742300003

EID of the result in the Scopus database

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